A custom-built microscope equipped with a 405 nm laser scanning system enabling targeted sample illumination. The primary application is induction of DNA damage within selected regions of the nucleus; however, the microscope could be used for other types of sample stimulation too. The system is based on an inverted microscope with a CMOS camera for widefield imaging. A 405 nm laser beam is scanned to position a sub-micron focus anywhere within a part of the objective’s field of view.

Available techniques:

• Brightfield and widefield epifluorescence imaging
• Controlled laser irradiation for inducing DNA damage or other sample stimulation

Objectives:

• LUCPLFLN 20x/0.45 dry, FWD 6.6-7.8 mm, CG 0-2.0 mm
  [FWD = free working distance, CG = cover glass]

Other Olympus objectives can be installed as required by the applications.

DNA damage laser line:

• 405 nm (laser power at the sample)

Light sources:

• halogen lamp (transmitted light)
• metal-halide lamp (epifluorescence

Filter sets:

• Laser illumination and reflected brightfield (---; 50/50 beamsplitter; 420LP)
• FITC epifluorescence (480/40; 510; 535/50)
• Cy3 epifluorescence (545/25; 565; 605/70)

Detectors and cameras:

• Basler daA3840-45um monochrome CMOS (3860x2178 pixels, 2 µm/pixel)

Software:

• Custom software to control the camera and define lines and speed of laser scanning

*Reduced rates apply:

• off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table

• long-term:
  

• 30% of the prevailing rate applicable after 10 hours of booking/usage

• 30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.

BACK TO TOP