NOBIC looks after imaging facilities at LKCMedicine and SCELSE, ensures the microscopes are in a good working order and troubleshoot technical issues. We train users in microscope operation, assist them with issues encountered during their experiments and collaborate with them on advanced imaging experiments and image analysis. Do not hesitate to contact us (nobic.facilities[..]e.ntu.edu.sg) to help you with any aspects of your imaging experiments. For more information visit:

Advanced Biofilm Imaging Facility (ABIF) at SCELSE

Advanced Optical Bio-Imaging Platform (AOBIP) at LKCMedicine

The imaging facilities are located in LKCMedicine Experimental Medicine Building (NTU Main Campus), Clinical Sciences Building (Novena Campus) and in SCELSE. See the details of each microscope for its location.

For New Users

To use the imaging facilities, register as in our equipment booking system (by registering as imaging facility user you accept the facility usage policies for ABIF and AOBIP). Usage of imaging facilities equipment requires training consisting of 3 parts:

Safety training required to work at LKCMedicine laboratories and/or SCELSE laboratories. If you plan to use a microscope containing lasers, you will also need NTU online laser safety modules (Non-ionizing Radiation: Introduction to NIR & local regulations - OHS2NRR01; Non-ionizing Radiation: Laser Safety Training - OHS2NRL01) and in some cases NEA N3 laser license (check with us if in doubt).
MyScope online modules developed by Microscopy Australia:
- Introduction to microscopy
- Work safety in a microscopy laboratory
- Light microscopy techniques (depending on the equipment you are planning to use)
  Kindly go through the relevant modules before the hands-on training and take the quiz in the last module (don’t worry it only concerns the most basic concepts covered by the modules).
Hands-on training; kindly submit a Training request in the booking system to identify which microscope(s) will work the best for you and plan the training.

Microscopes

Key to microscope quick details (location / fluorophores & filters / objectives):

Location: Experimental Medicine Building (E), Clinical Sciences Building (C) and SCELSE (S).

Fluorophores & filters (for fluorophore spectra refer e.g. to FPbase):

by exc. wavelength: (1) DAPI-like ( ≤ 405 nm), (2) CFP-like (420-460 nm), (3) FITC/GFP-like (470-490 nm), (4) YFP-like (500-520 nm), (5) Cy3/DsRed-like (530-560 nm), (6) mCherry/A568-like (560-590 nm), (7) Cy5-like (620-650 nm), (8) cy7-like (>650 nm)
(F) fixed exc and em. filter pairs, (F) (at least partially) flexible combination of exc. and em., (F) freely selectable emission range/spectral detection

Objectives:

by magnification: (A) < 4x, (A) 4-5x, (A) 10x, (A) 16-32x, (A) 40-50x, (A) 60-75x, (A) 100-150x
by immersion & working distance: (A) air, (W), (O) oil, (A) long working distance (≥ 2 mm or compatible with regular plastic-bottom dishes)
- Carl Zeiss Axio Observer Z1 – inverted epifluorescence microscope (S / 134567 / 4A20A40A63A63O100O)
- Carl Zeiss Axio Imager M2 – upright epifluorescence microscope (S / 13456 / 10A20A40O63O100O)
- Nikon Eclipse Ts2 FL – routine inverted epifluorescence microscope (S / 135 / 10A20A40A)
- Nikon Eclipse Ti-E - inverted epifluorescence microscope (E / 13567 / 4A10A20A40A60A)
- Leica DM5500 - upright epifluorescence microscope (E / 135 / 1.25A5A10A20A40A63O)
- Carl Zeiss Axio Imager M2 - upright epifluorescence microscope (E / 1234567 / 4A10A20A40W63W63O)
- Carl Zeiss Axio Observer 7 with Aurox Clarity - inverted widefield microscope with a laser-free spinning disk module (S / Widefield: 13567 / 10A40A63A100O)
- Carl Zeiss Cell Discoverer 7 - inverted widefield fluorescence microscope in a closed box (E / 123467 / 5A20A20A50W)
- GE IN Cell Analyzer 2200 - high-content screening widefield fluorescence microscope (E / 1234567 / 4A10A20A40A)
- Perkin-Elmer Operetta CLS - high-content screening widefield and confocal (spinning disk) microscope (C / 1234578 / 5A10A20A20W40W63W)
- Carl Zeiss Axio Observer 7 - inverted live-cell widefield fluorescence microscope (C / 1234567 / 10A10A20A40A40O63O)
- Leica DMI6000 - inverted live-cell widefield fluorescence microscope (C)
- Leica DMI6000 - inverted widefield microscope for Calcium imaging (C)
- Carl Zeiss LSM 780 – laser scanning confocal with fast spectral detection (32-GaAsP array) (S / 1234567 / 20A20A40A40O63O)
- Carl Zeiss ELYRA PS.1 with LSM 780 confocal – super-resolution (SIM, PALM, STORM, …) and TIRF microscope combined with a laser scanning confocal (S / confocal: 1234567 / 10A20A40O63W63O100O)
- Carl Zeiss Axio Observer 7 with Aurox Clarity - inverted widefield microscope with a laser-free spinning disk module (S / Spinning disk: 134567 / 10A40A63A100O)
- Carl Zeiss LSM 800 - inverted confocal with Airy scan super-resolution capability (E / 13567 / 10A20A20A40O63O)
- Carl Zeiss LSM 800 - inverted confocal with Airy scan super-resolution capability and live-cell accessories (C / 13567 / 10A20A40A40O63W63O)
- Nikon Ti-E with Yokogawa CSU-W1 spinning disk - motorised inverted spinning disk confocal with 50 μm pinholes and instant super-resolutiion capability (C)
- Perkin-Elmer Operetta CLS - high-content screening widefield and confocal (spinning disk) microscope (C / 1234578 / 5A10A20A20W40W63W)
- Carl Zeiss LSM 800 - upright confocal (E / 13567 / 5A10A20W40W63W)
- Leica SPE - upright confocal (C)
- Leica SP8 - upright confocal microscope with micromanipulator and micro-injection rig (C)
- Carl Zeiss Lightsheet Z.1 - multi-view lightsheet microscope (E / 23456 / 5A20W)
- Carl Zeiss Lightsheet Z.1 - lightsheet microscope with a cleared tissue add-on (C)
- Carl Zeiss ELYRA PS.1 with LSM 780 confocal – super-resolution (SIM, PALM, STORM, …) and TIRF microscope combined with a laser scanning confocal (S / TIRF and SR: 13567 / 10A20A40O63W63O100O)
- Nikon N-STORM - super-resolution (PALM, STORM, …) and TIRF microscope with FRAP capability (C)
- Bruker Prairie Ultima - upright multiphoton microscope with 405 nm and 561 nm lasers for uncaging (C)
- Upright multi-photon microscope with FLIM capability - upright microscope with tunable IR laser (multi-photon) and visible lasers (confocal) and time-resolved detection (FLIM) (C)
- Upright multi-photon microscope for optogenetics - upright microscope with 2 IR lasers (multi-photon imaging and optogenetics) and visible lasers (confocal) (C)
- Multi-photon mesoscope - upright system for multi-photon imaging of large (5 mm diameter) fields of view (C)
- GE IN Cell Analyzer 2200 - high-content screening widefield fluorescence microscope (E / 1234567 / 4A10A20A40A)
- Perkin-Elmer Operetta CLS - high-content screening widefield and confocal (spinning disk) microscope (C / 1234578 / 5A10A20A20W40W63W)
- Carl Zeiss Axio Scan.Z1 - slide scanner for transmitted light and fluorescence imaging (C / 1235678 / 5A5A10A20A40A)
- Custom Raman and Brillouin microspectroscopy setup (S)
- Carl Zeiss Discovery.V8 – parallel (CMO) stereo microscope with an RGB camera (S)
- Carl Zeiss Axio Zoom V.16 – parallel (CMO) stereo microscope with epifluorescence capability and an RGB camera (E / 135)
- Leica S6 D - Greenough stereo microscope with an RGB camera (E)
Microscope-Fluorophore Selector

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For New Users

Microscopes

Microscope-Fluorophore Selector