NOBIC looks after imaging facilities at LKCMedicine and SCELSE, ensures the microscopes are in a good working order and troubleshoot technical issues. We train users in microscope operation, assist them with issues encountered during their experiments and collaborate with them on advanced imaging experiments and image analysis. Do not hesitate to contact us (nobic.facilities[..]e.ntu.edu.sg) to help you with any aspects of your imaging experiments. For more information visit:

Advanced Biofilm Imaging Facility (ABIF) at SCELSE

Advanced Optical Bio-Imaging Platform (AOBIP) at LKCMedicine

The imaging facilities are located in LKCMedicine Experimental Medicine Building (NTU Main Campus), Clinical Sciences Building (Novena Campus) and in SCELSE. See the details of each microscope for its location.

For New Users

To use the imaging facilities, register as in our equipment booking system (by registering as imaging facility user you accept the facility usage policies for ABIF and AOBIP). Usage of imaging facilities equipment requires training consisting of 3 parts:

Safety training required to work at LKCMedicine laboratories and/or SCELSE laboratories. If you plan to use a microscope containing lasers, you will also need NTU online laser safety modules (Non-ionizing Radiation: Introduction to NIR & local regulations - OHS2NRR01; Non-ionizing Radiation: Laser Safety Training - OHS2NRL01) and in some cases NEA N3 laser license (check with us if in doubt).
MyScope online modules developed by Microscopy Australia:
- Introduction to microscopy
- Work safety in a microscopy laboratory
- Light microscopy techniques (depending on the equipment you are planning to use)
  Kindly go through the relevant modules before the hands-on training and take the quiz in the last module (don’t worry it only concerns the most basic concepts covered by the modules).
Hands-on training; kindly submit a Training request in the booking system to identify which microscope(s) will work the best for you and plan the training.

Microscopes

Key to microscope locations: Experimental Medicine Building (E), Clinical Sciences Building (C) and SCELSE(S).

Carl Zeiss Axio Observer Z1 – inverted epifluorescence microscope (S)

Carl Zeiss Axio Imager M2 – upright epifluorescence microscope (S)

Nikon Eclipse Ts2 FL – routine inverted epifluorescence microscope (S)

Nikon Eclipse Ti-E - inverted epifluorescence microscope (E)

Leica DM5500 - upright epifluorescence microscope (E)

Carl Zeiss Axio Observer 7 with Aurox Clarity - inverted widefield microscope with a laser-free spinning disk module (S)
Systems at AOBIP

Leica TCS SP8 X WLL – laser scanning confocal with a white light laser and spectral detection (S)

Carl Zeiss LSM 780 – laser scanning confocal with fast spectral detection (32-GaAsP array) (S)

Carl Zeiss ELYRA PS.1 with LSM 780 confocal – super-resolution (SIM, PALM, STORM, …) and TIRF microscope combined with a laser scanning confocal (S)

Carl Zeiss Axio Observer 7 with Aurox Clarity - inverted widefield microscope with a laser-free spinning disk module (S)

Systems at AOBIP

Systems at AOBIP

Systems at AOBIP

Carl Zeiss ELYRA PS.1 with LSM 780 confocal – super-resolution (SIM, PALM, STORM, …) and TIRF microscope combined with a laser scanning confocal (S)
Systems at AOBIP

Systems at AOBIP

High-content screening microscopes at AOBIP

Slide-scanner at AOBIP

Custom Raman and Brillouin microspectroscopy setup – stereomicroscope with an RGB camera (S)

Calcium imaging system at AOBIP

Carl Zeiss Discovery.V8 – stereomicroscope with an RGB camera (S)