NOBIC looks after imaging facilities at LKCMedicine and SCELSE, ensures the microscopes are in a good working order and troubleshoot technical issues. We train users in microscope operation, assist them with issues encountered during their experiments and collaborate with them on advanced imaging experiments and image analysis. Do not hesitate to contact us (nobic.facilities[..]e.ntu.edu.sg) to help you with any aspects of your imaging experiments. For more information visit:

Advanced Biofilm Imaging Facility (ABIF) at SCELSE

Advanced Optical Bio-Imaging Platform (AOBIP) at LKCMedicine

The imaging facilities are located in LKCMedicine Experimental Medicine Building (NTU Main Campus), Clinical Sciences Building (Novena Campus) and in SCELSE. See the details of each microscope for its location.

Important Note

When publishing (in the form of journal articles, talks, posters, social media posts etc.) any images obtained at NOBIC facilities and/or any results derived from those images, kindly acknowledge the facility. Suggested acknowledgment text follows:

"Microscopy images were acquired at NTU Optical Bio-Imaging Centre (NOBIC) imaging facilities at LKCMedicine*/SCELSE*."
(*select whichever applies)

In case NOBIC staff assisted you in your experiment or image processing and analysis, kindly add:

"Assistance of NOBIC staff with image acquisition* / processing* / analysis* is acknowledged." (*select whichever applies)

Do not hesitate to contact us if you need advice on describing the microscopy experiment in Materials & Methods (or analogous) section of your manuscript to ensure the description contains all details needed to reproduce the experiment.

For New Users

To use the imaging facilities, register in our equipment booking system (by registering as imaging facility user you accept the facility usage policies for ABIF and SOPs for microscope booking, utilisation monitoring, incident reporting and equipment maintenance applicable to AOBIP). When using the facilities, kindly follow these essential guidelines. Usage of imaging facilities equipment requires training consisting of 3 parts:

• Safety training required to work at LKCMedicine laboratories and/or SCELSE laboratories. If you plan to use a microscope containing lasers, you will also need NTU online laser safety module (Working with Non-Ionising Radiation - OHS2NIR01) and in some cases NEA N3 laser license (check with us if in doubt).
  
  
• MyScope online modules developed by Microscopy Australia:
  • Introduction to microscopy
  • Work safety in a microscopy laboratory
  • Light microscopy techniques - depending on the equipment you are planning to use (e.g. if you will be applying for confocal microscopy training, take options 1. - 3.)
    1. Basics and Brightfield
    2. Fluorescence Microscopy
    3. Confocal Microscopy
    4. Super-resolution Microscopy (basics)
    5. Super-resolution - Localisation Microscopy
    Kindly go through the relevant modules before the hands-on training, take the quiz in the last module (don’t worry it only concerns the most basic concepts covered by the modules) and share with us your success. Completing the quiz is a prerequisite for the hands-on training and you will be asked to submit the quiz result in the Training request.
    
    
• Hands-on training; kindly submit a Training request in the booking system to identify which microscope(s) will work the best for you and plan the training.

Microscopes

Key to microscope quick details (location / fluorophores & filters / objectives):

Location: Experimental Medicine Building (E), Clinical Sciences Building (C) and SCELSE (S).

Fluorophores & filters (for fluorophore spectra refer e.g. to FPbase):

• by exc. wavelength: (1) DAPI-like ( ≤ 405 nm), (2) CFP-like (420-460 nm), (3) FITC/GFP-like (470-490 nm), (4) YFP-like (500-520 nm), (5) Cy3/DsRed-like (530-560 nm), (6) mCherry/A568-like (560-590 nm), (7) Cy5-like (620-650 nm), (8) Cy7-like (>650 nm)
• (F) fixed exc. and em. filter pairs, (F) (at least partially) flexible combination of exc. and em., (F) freely selectable emission range/spectral detection

Objectives:

• by magnification: (A) < 4x, (A) 4-5x, (A) 10x, (A) 16-32x, (A) 40-50x, (A) 60-75x, (A) 100-150x
• by immersion & working distance: (A) air, (W) water, (O) oil, (S) silicone, (A) long working distance (≥ 2 mm or compatible with regular plastic-bottom dishes)
  
  Epifluorescence microscopes
  
  
  • Carl Zeiss Axio Observer Z1 – inverted epifluorescence microscope (S / 134567 / 4A20A40A63A63O100O)
  
  
  • Carl Zeiss Axio Imager M2 – upright epifluorescence microscope (S / 1356 / 10A20A40A40O63O100O)
  
  
  • Nikon Eclipse Ts2 FL – routine inverted epifluorescence microscope (S / 135 / 10A20A40A)
  
  
  • Nikon Eclipse Ti-E - inverted epifluorescence microscope (E / 13567 / 4A10A20A40A60A)
  
  
  • Leica DM5500 - upright epifluorescence microscope (E / 135 / 1.25A5A10A20A40A63O)
  
  
  • Carl Zeiss Axio Imager M2 - upright epifluorescence microscope (E / 1234567 / 4A10A20A40W63W63O)
  
  
  Live-cell epifluorescence (inverted) microscopes
  
  
  • Carl Zeiss Axio Observer 7 with Aurox Clarity - inverted widefield microscope with a laser-free spinning disk module (S / Widefield: 13567 / 10A40A63A63O100O)
  
  
  • Carl Zeiss Cell Discoverer 7 - inverted widefield fluorescence microscope in a closed box (E / 123467 / 5A20A50W)
  
  
  • GE IN Cell Analyzer 2200 - high-content screening widefield fluorescence microscope (E / 13567 / 4A10A20A40A)
  
  
  • Perkin-Elmer Operetta CLS - high-content screening widefield and confocal (spinning disk) microscope (C / 1234578 / 5A10A20A20W40W63W)
  
  
  • Carl Zeiss Axio Observer 7 - inverted live-cell widefield fluorescence microscope (C / 1234567 / 2.5A10A20A40A40O63O)
  
  
  Inverted confocal microscopes
  
  

  • Carl Zeiss LSM 780 – laser scanning confocal with fast spectral detection (32-GaAsP array) (S / 1234567 / 20A20A40A40O63O)
  
  
  • Carl Zeiss ELYRA PS.1 with LSM 780 confocal – super-resolution (SIM, PALM, STORM, …) and TIRF microscope combined with a laser scanning confocal (S / confocal: 1234567 / 20A40O63A63W100O)
  
  
  • Olympus FV1200 – laser scanning confocal microscope (S / 13567 / 10A20A30S63O100O)
  
  
  • Carl Zeiss Axio Observer 7 with Aurox Clarity - inverted widefield microscope with a laser-free spinning disk module (S / Spinning disk: 134567 / 10A40A63A63A100O)
  
  
  • Carl Zeiss LSM 800 - inverted confocal with Airy scan super-resolution capability (E / 13567 / 2.5A10A20A40O63W63O)
  
  
  • Carl Zeiss LSM 800 - inverted confocal with Airy scan super-resolution capability and live-cell accessories (C / 13567 / 10A20A25WSGO40A40O63W63O)
  
  
  • Nikon AX R - inverted confocal with resonant scanner, NSPRC super-resolution and live-cell accessories (C / 1235678 / 2A10A20A20W25S40A40W40S63O)
  
  
  • Perkin-Elmer Operetta CLS - high-content screening widefield and confocal (spinning disk) microscope (C / 1234578 / 5A10A20A20W40W63W)
  
  
  Upright confocal microscopes
  
  
  • Carl Zeiss LSM 800 - upright confocal (E / 13567 / 5A10A20W40W63W)
  
  
  • Leica SP8 - upright confocal microscope and stereo microscope at Animal Research Facility (C / 135678)
  
  
  Lightsheet microscopes
  
  

  • Carl Zeiss Lightsheet Z.1 - multi-view lightsheet microscope (E / 23456 / 5A20W63W)
  
  
  • Carl Zeiss Lightsheet Z.1 - lightsheet microscope with a cleared tissue chamber (RI = 1.45) (C / 234567 / 5A20W20clearing )
  
  
  TIRF and Super-resolution microscopes
  
  
  • Carl Zeiss ELYRA PS.1 with LSM 780 confocal – super-resolution (SIM, PALM, STORM, …) and TIRF microscope combined with a laser scanning confocal (S / TIRF and SR: 13567 / 20A40O63A63W100O)
  
  
  Multi-photon microscopes
  
  
  • 2-photon microscope Guss - custom 2-photon microscope for SHG imaging (S / 6 / 40W)
  
  
  • 2-photon microscope Nemo - custom 2-photon microscope for optogenetics (E / 35 / 20W)
  
  
  • 2-photon microscope Stuart - custom 2-photon microscope (C / 35 / 20W)
  
  
  • 2-photon microscope FVMPE-RS - Olympus FVMPE-RS 2-photon microscope with FLIM module (C / 123456 / 20W)
  
  
  High-throughput microscopes
  
  

  • Carl Zeiss Cell Discoverer 7 - inverted widefield fluorescence microscope in a closed box (E / 123467 / 5A20A50W)
  
  
  • GE IN Cell Analyzer 2200 - high-content screening widefield fluorescence microscope (E / 13567 / 4A10A20A40A)
  
  
  • Perkin-Elmer Operetta CLS - high-content screening widefield and confocal (spinning disk) microscope (C / 1234578 / 5A10A20A20W40W63W)
  
  
  • Carl Zeiss Axio Scan.Z1 - slide scanner for transmitted light and fluorescence imaging (C / 1235678 / 5A5A10A20A40A)
  
  
  Specialised microscopes
  
  
  • Custom Raman and Brillouin microspectroscopy setup (S / 4A20A60O)
  
  
  Stereo microscopes
  
  

  • Carl Zeiss Discovery.V8 – parallel (CMO) stereo microscope with an RGB camera (S)
  
  
  • Carl Zeiss Axio Zoom V.16 – parallel (CMO) stereo microscope with epifluorescence capability and an RGB camera (E / 135)
  
  
  • Leica S6 D - Greenough stereo microscope with an RGB camera (E)
  
  
  

  Microscope-Fluorophore Selector

  Excitation: (XXX nm) laser line of XXX nm, (F) filter set with fixed exc. and em. filter pair, (F) (at least partially) flexible combination of exc. and em. filters, (F) freely selectable emission range/spectral detection

  Objectives: (A) air, (W), (O) oil, (S) silicone, (G) glycerine, (A) long working distance (≥ 2 mm or compatible with regular plastic-bottom dishes)

  flakonda

  DAPI-like ( ≤ 405 nm)

  CFP-like (420-460 nm)

  FITC/GFP-like (470-490 nm)

  YFP-like (500-520 nm)

  Cy3/ DsRed-like (530-560 nm)

  mCherry/ A568-like (560-590 nm)

  Cy5-like (620-650 nm)

  Cy7-like (>650 nm)

  < 4x

  4-5x

  10x

  16-32x

  40-50x

  60-75x

  100-150x

  flakonda

  Microscope

  Excitation

  Objectives

  Microscope

  CSB

  DAPI-like ( ≤ 405 nm)

  CFP-like (420-460 nm)

  FITC/GFP-like (470-490 nm)

  YFP-like (500-520 nm)

  Cy3/ DsRed-like (530-560 nm)

  mCherry/ A568-like (560-590 nm)

  Cy5-like (620-650 nm)

  Cy7-like (>650 nm)

  < 4x

  4-5x

  10x

  16-32x

  40-50x

  60-75x

  100-150x

  CSB

  2-photon microscope Stuart

  F

  F

  20W

  2-photon microscope Stuart

  2-photon microscope FVMPE-RS - Olympus FVMPE-RS with FLIM module

  F

  F

  F

  F

  F

  F

  20W

  2-photon microscope FVMPE-RS - Olympus FVMPE-RS with FLIM module

  Perkin-Elmer Operetta CLS - high-content screening widefield spinning disk

  F

  F

  F

  F

  F

  F

  F

  5A

  10A

  20A, 20 W

  40W

  63W

  Perkin-Elmer Operetta CLS - high-content screening widefield spinning disk

  Carl Zeiss Axio Observer 7 - inverted live-cell widefield

  F

  F

  F

  F

  F

  F

  F

  2.5A

  10A

  20A

  40A, 40O

  63O

  Carl Zeiss Axio Observer 7 - inverted live-cell widefield

  Carl Zeiss LSM 800 - inverted live-cell confocal with Airy scan

  405 nm

  488 nm

  561 nm

  640 nm

  10A

  20A, 25WSGO

  40A, 40O

  63W, 63O

  Carl Zeiss LSM 800 - inverted live-cell confocal with Airy scan

  Nikon AX R - inverted live-cell confocal with resonant scanner and NSPARC

  405 nm

  445 nm

  488 nm

  561 nm

  640 nm

  730 nm

  2A

  10A

  20A, 20W, 20S

  40A, 40W, 40S

  60O

  Nikon AX R - inverted live-cell confocal with resonant scanner and NSPARC

  Leica SP8 - upright confocal at ARF

  405 nm

  470 - 670 nm WLL

  single objective installed at a time; refer to microscope page for a list of objectives

  Leica SP8 - upright confocal at ARF

  Carl Zeiss Lightsheet Z.1 - lightsheet with cleared tissue chamber

  445 nm

  488 nm

  515 nm

  561 nm

  638 nm

  5A

  20W, 20(clearing)

  Carl Zeiss Lightsheet Z.1 - lightsheet with cleared tissue add-on

  Carl Zeiss Axio Scan.Z1 - slide scanner

  F

  F

  F

  F

  F

  F

  F

  5A, 5A

  10A

  20A

  40A

  Carl Zeiss Axio Scan.Z1 - slide scanner

  EMB

  DAPI-like ( ≤ 405 nm)

  CFP-like (420-460 nm)

  FITC/GFP-like (470-490 nm)

  YFP-like (500-520 nm)

  Cy3/ DsRed-like (530-560 nm)

  mCherry/ A568-like (560-590 nm)

  Cy5-like (620-650 nm)

  Cy7-like (>650 nm)

  < 4x

  4-5x

  10x

  16-32x

  40-50x

  60-75x

  100-150x

  EMB

  2-photon microscope Nemo - custom 2-photon optogenetic microscope

  F

  F

  20W

  2-photon microscope Nemo - custom 2-photon optogenetic microscope

  GE IN Cell Analyzer 2200 - high-content screening widefield

  F

  F

  F

  F

  F

  4A

  10A

  20A

  40A

  GE IN Cell Analyzer 2200 - high-content screening widefield

  Carl Zeiss LSM 800 - inverted confocal with Airy scan

  405 nm

  488 nm

  561 nm

  640 nm

  2.5A

  10A

  20A

  40O

  63W, 63O

  Carl Zeiss LSM 800 - inverted confocal with Airy scan

  Carl Zeiss Cell Discoverer 7 - inverted live-cell widefield

  F

  F

  F

  F

  F

  F

  F

  5A

  20A

  50W

  Carl Zeiss Cell Discoverer 7 - inverted live-cell widefield

  Carl Zeiss Lightsheet Z.1 - multi-view lightsheet

  445 nm

  488 nm

  515 nm

  561  nm

  5A

  20W

  63W

  Carl Zeiss Lightsheet Z.1 - multi-view lightsheet

  Nikon Eclipse Ti-E - inverted epifluor. microscope

  F

  F

  F

  F

  4A

  10A

  20A

  40A

  60A

  Nikon Eclipse Ti-E - inverted epifluor. microscope

  Carl Zeiss LSM 800 - upright confocal

  405 nm

  488 nm

  561 nm

  640 nm

  5A

  10A

  20W

  40W

  63W

  Carl Zeiss LSM 800 - upright confocal

  Leica DM5500 - upright epifluor. microscope

  F

  F

  F

  1.25A

  5A

  10A

  20A

  40A

  63O

  Leica DM5500 - upright epifluor. microscope

  Carl Zeiss Axio Imager M2 - upright epifluor. microscope

  F

  F

  F

  F

  F

  F

  F

  5A

  10A

  20A

  40W

  63W, 63O

  Carl Zeiss Axio Imager M2 - upright epifluor. microscope

  Carl Zeiss Axio Zoom V.16 –  stereo with a camera

  F

  F

  F

  Carl Zeiss Axio Zoom V.16 –  stereo with a camera

  SCELSE

  DAPI-like ( ≤ 405 nm)

  CFP-like (420-460 nm)

  FITC/GFP-like (470-490 nm)

  YFP-like (500-520 nm)

  Cy3/ DsRed-like (530-560 nm)

  mCherry/ A568-like (560-590 nm)

  Cy5-like (620-650 nm)

  Cy7-like (>650 nm)

  < 4x

  4-5x

  10x

  16-32x

  40-50x

  60-75x

  100-150x

  SCELSE

  2-photon microscope Guss - custom 2-photon microscope for SHG

  F

  40W

  2-photon microscope Guss - custom 2-photon microscope for SHG

  Nikon Eclipse Ts2 FL – routine inverted epifluor. microscope

  F

  F

  F

  10A

  20A

  40A

  Nikon Eclipse Ts2 FL – routine inverted epifluor. microscope

  Carl Zeiss Axio Imager M2– upright epifluor. microscope

  F

  F

  F

  F

  10A

  20A

  40A, 40O

  63O

  100O

  Carl Zeiss Axio Imager M2– upright epifluor. microscope

  Carl Zeiss Axio Observer 7 - inverted widefield with with Aurox Clarity laser-free spinning disk

  F

  F

  F

  F

  10A

  40A

  63A, 63O

  100O

  Carl Zeiss Axio Observer 7 - inverted widefield with with Aurox Clarity laser-free spinning disk

  F

  F

  F

  F

  F

  F

  10A

  40A

  63A

  100O

  Carl Zeiss Axio Observer Z1 – inverted epifluor. microscope

  F

  F

  F

  F

  F

  4A

  20A

  40A

  63A, 63O

  100O

  Carl Zeiss Axio Observer Z1 – inverted epifluor. microscope

  Carl Zeiss ELYRA PS.1 (SIM, PALM, STORM, …) and TIRF with LSM 780 inverted confocal

  405 nm

  488 nm

  561nm

  642 nm

  20A

  40O

  63A, 63W

  100O

  Carl Zeiss ELYRA PS.1 (SIM, PALM, STORM, …) and TIRF with LSM 780 inverted confocal

  405 nm

  458 nm

  488 nm

  514 nm

  561 nm

  633 nm

  Carl Zeiss LSM 780 – inverted confocal

  405 nm

  458 nm

  488 nm

  514 nm

  561 nm

  633 nm

  20A, 20A

  40A, 40O

  63O

  100O

  Carl Zeiss LSM 780 – inverted confocal

  Olympus FV1200 – inverted confocal

  405 nm

  473 nm

  558 nm

  635 nm

  10A

  20A, 30S

  63O

  100O

  Olympus FV1200 – inverted confocal

  
  
  
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