GE IN Cell Analyzer 2200 is a high throughput widefield fluorescence microscope with sensitive sCMOS camera and a broad range of fluorescence filters. Sample temperature, (potentially CO2) and humidity control allow long term experiments; hardware autofocus ensures reliable focusing during long-term experiments. The image acquisition software contains image deconvolution functionalities.
Available techniques:
- Transmitted light imaging (brightfield, DIC, computational phase contrast - constructed from in-focus and 2 out-of-focus images)
- Widefield multi-channel fluorescence imaging with a fast and sensitive camera
- Long-term time-lapse imaging (with temperature control and hardware autofocus)
- Fast multi-well plate screening
Objectives:
- Plan Apo 4x/0.2 dry, FWD 15.7 mm, CG -
- Plan Apo 10x/0.45 dry, FWD 4.0 mm, CG 0.17 mm (DIC)
- Plan Fluor ELWD 20x/0.45 dry, FWD 8.1 - 7.0 mm, CG 0 - 2 mm (DIC)
- Plan Apo 40x/0.95 dry, FWD 0.14 mm, CG 0.11 - 0.23 mm (DIC)
[FWD = working distance, CG = cover glass]
Light sources:
- LED lamp (fluorescence)
- LED lamp (transmitted light)
Filter sets:
Excitation filters:
- DAPI (390/18)
- CFP (438/24)
- FITC (475/28)
- YFP (513/17)
- Cy3 (542/27)
- TexasRed (575/25)
- Cy5 (632/22)
Dichroic beamsplitters:
- Quad-band (DAPI + FITC + Cy3 + Cy5)
- Quad-band (DAPI + FITC + TexasRed + Cy5)
Emission filters:
- DAPI (435/48)
- CFP (475/24)
- FITC (511/23)
- FITC (525/48)
- YFP (548/22)
- Cy3 (597/45)
- TexasRed (620/30)
- Cy5 (679/34)
Detectors and cameras:
- sCMOS monochrome camera
Software:
- IN Cell Investigator
Other features:
- Motorised stage
- Motorised focus drive
- Motorised filter turret
- Temperature control
- CO2 concentration control only with user-supplied 25% CO2/air mixture cylinder
- Hardware autofocus
| Usage fees [SGD/hour] | LKCMed | NTU | Others (Academia/Industry) |
| 10* | 20 | 30 / 48 | |
| Location | EMB 07-07-01, Imaging room (apply for card access) | ||
| Contact | nobic.facilities@e.ntu.edu.sg | ||
*Reduced rates apply:
- off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table
- long-term:
- 30% of the prevailing rate applicable after 10 hours of booking/usage
- 30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.