This motorised inverted widefield fluorescence microscope features a fast and sensitive sCMOS camera for fluorescence detection and a colour camera for transmitted light. Hardware autofocus ensures continuously stable focusing throughout long imaging experiments. The system is suitable for a wide variety of widefield imaging experiments (brightfield, phase contrast, multi-channel fluorescence) on live or fixed samples.
Available techniques:
- Transmitted light imaging (brightfield, phase contrast)
- Widefield multi-channel fluorescence imaging with a fast and sensitive camera
- Long-term time-lapse imaging (with hardware autofocus)
Objectives:
- CFI Plan Fluor 4x/0.13 dry, FWD 17.2 mm, CG - (Phase contrast)
- CFI Plan Fluor 10x/0.3 dry, FWD 16 mm, CG 0.17 mm (Phase contrast)
- CFI S Plan Fluor ELWD 20x/0.45 dry, FWD 8.2 - 6.9 mm, CG 0 - 2 mm (Phase contrast)
- CFI S Plan Fluor ELWD 40x/0.6 dry, FWD 2.8 - 3.6 mm, CG 0 - 2 mm (Phase contrast)
- CFI S Plan Fluor ELWD 60x/0.7 dry, FWD 1.8 - 2.6 mm, CG 0.1 - 1.3 mm
(Phase contrast)
[FWD = free working distance, CG = cover glass]
Fluorescence excitation sources:
- X-Cite 120 LED
Filter sets:
- DAPI (377/50; 409 ; 447/60)
- GFP (466/40; 495; 525/50)
- mCherry (562/40; 593; 641/75)
- Cy5 (628/40; 660; 692/40)
Detectors and cameras:
- Hamamatsu Orca Flash 4.0 V2 sCMOS (2048x2048 pixels, 6.5 µm/pixel)
- Nikon DS-Ri 1 colour CCD camera (1280x1024 pixels, 6.45 µm/pixel)
Software:
- MetaMorph
- NIS Elements D
- Micro-Manager 2.0
Other features:
- Long-working distance condenser N.A. 0.52 W.D. 30 mm
- Manual stage
- Motorised filter turret
- Motorised focus drive
- PFS hardware autofocus
| Usage fees [SGD/hour] | LKCMed | NTU | Others (Academia/Industry) |
| 0 | 15 | 30 / 48 | |
| Location | EMB 03-02Y | ||
| Contact | nobic.facilities@e.ntu.edu.sg | ||