This lightsheet microscope is equipped with pivot scan for 3D imaging, a sensitive sCMOS camera and sample temperature control. Beside optics for imaging specimens in water, optics for imaging cleared specimens of refractive index n = 1.45 are also available. A camera and an IR LED (to minimise phototoxicity) in the sample chambre facilitate sample positioning.
Objectives:
Excitation (lightsheet formation):
- 5x/0.1
- 10x/0.2
Imaging:
Note that only a single objective lens can be installed in the microscope at a time. Refer to the booking system for current configuration; you may request different objective when booking the microscope.
- EC Plan-Neofluar 5x/0.16, WD 5.1 mm, for water immersion
- W Plan-Apochromat 20x/1.0 water immersion, WD 2.4 mm
- Clr Plan-Neofluar 20x/1.0 Corr n = 1.45 clearing immersion,
WD 5.6 mm
[WD = working distance]
Fluorescence excitation lasers:
- 445 nm
- 488 nm
- 515 nm
- 561 nm
- 638 nm
Fluorescence filters:
Note that only a single filter turret can be installed in the microscope at a time. Refer to the booking system for current configuration; you may request different objective when booking the microscope.
Emission filters turret 1:
- GFP (LP 510)
- YFP (BP 525-565)
- dsRed (LP 580)
- mCherry (LP 585)
- DRAQ5 (LP 660)
Emission filters turret 2:
- CFP (BP 460-500)
- CFP/GFP (LP 490)
- GFP (LP 510)
- GFP (BP 505-545)
- Cy3 (BP 575-615)
[BP = band pass, LP = long pass]
Detectors and cameras:
- PCO.edge (1920x1920 pixels, 6.5 µm/pixel)
Software:
- ZEN Blue
Other features:
- temperature control
- chambre for imaging cleared specimen with refractive index n = 1.45
| Usage fees [SGD/hour] | LKCMed | NTU | Others (Academia/Industry) |
| 25 | 50 | 60 / 96 | |
| Location | CSB 11-02P-01 | ||
| Contact | nobic.facilities@e.ntu.edu.sg | ||