This lightsheet microscope is equipped with illumination optics on two sides for homogeneous sample excitation, pivot scan for 3D imaging, 2 sensitive sCMOS cameras for simultaneous 2-channel imaging and environmental control for live specimens (temperature and CO2 concentration). A camera and an IR LED (to minimise phototoxicity) in the sample chamber facilitate sample positioning.
Note that only a single objective lens can be installed in the microscope at a time.
Note that the filter sets do not separate excitation from emission paths; it splits fluorescence onto the two cameras for 2-channel imaging. The filter sets consist of emission filter 1; secondary beamsplitter; emission filter 2.
|Usage fees [SGD/hour]||NTU||Others|
|Location||EMB 04-02Y, Zebrafish facility|