This lightsheet microscope is equipped with illumination optics on two sides for homogeneous sample excitation, pivot scan for 3D imaging, 2 sensitive sCMOS cameras for simultaneous 2-channel imaging and environmental control for live specimens (temperature and CO2 concentration). A camera and an IR LED (to minimise phototoxicity) in the sample chamber facilitate sample positioning.

Objectives:

Excitation (lightsheet formation):

Imaging:

Note that only a single objective lens can be installed in the microscope at a time.

Fluorescence excitation lasers:

Fluorescence filter sets:

Note that the filter sets do not separate excitation from emission paths; it splits fluorescence onto the two cameras for 2-channel imaging. The filter sets consist of emission filter 1; secondary beamsplitter; emission filter 2.

Detectors and cameras:

Software:

Other features:

Usage fees [SGD/hour] NTU Others
60 75
Location EMB 04-02Y, Zebrafish facility
Contact nobic.facilities@e.ntu.edu.sg, kbala@ntu.edu.sg

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