This lightsheet microscope is equipped with illumination optics on two sides for homogeneous sample excitation, pivot scan for 3D imaging, 2 sensitive sCMOS cameras for simultaneous 2-channel imaging and environmental control for live specimens (temperature). A camera and an IR LED (to minimise phototoxicity) in the sample chamber facilitate sample positioning.

Objectives:

Excitation (lightsheet formation):

5x/0.1
10x/0.2

Imaging:

Note that only a single objective lens can be installed in the microscope at a time. Refer to the booking system for current configuration; you may request different objective when booking the microscope.

EC Plan-Neofluar 5x/0.16, WD 5.1 mm, for water immersion
W Plan-Apochromat 20x/1.0 water immersion, WD 2.4 mm
W Plan-Apochromat 63x/1.0 water immersion, WD 2.1 mm
[WD = working distance]

Fluorescence excitation lasers:

445 nm
488 nm
515 nm
561 nm

Fluorescence filter sets:

Note that the filter sets do not separate excitation from emission paths; it splits fluorescence onto the two cameras for 2-channel imaging. The filter sets consist of emission filter 1; secondary beamsplitter; emission filter 2.

CFP/YFP (BP 460-500; LP 510; BP 525-565)
CFP/mCherry (BP 460-500; LP 560; LP 585)
GFP/mCherry (BP 505-545; LP 560; LP 585)
YFP/mCherry (BP 525-565; LP 580; LP 585)
[BP = band pass, LP = long pass]

Detectors and cameras:

2x PCO.edge (1920x1920 pixels, 6.5 µm/pixel)

Software:

ZEN Blue

Other features:

temperature control

Usage fees [SGD/hour]	LKCMed	NTU	Others (Academia/Industry)
Usage fees [SGD/hour]	25*	50	60 / 96
Location	EMB 04-22H-01, Zebrafish facility (apply for card access)
Contact	nobic.facilities@e.ntu.edu.sg

*Reduced rates apply:

off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table

long-term:

30% of the prevailing rate applicable after 10 hours of booking/usage

30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.