Thorlabs 2-photom Random Access Mesoscope is designed primarily for 2-photon in-vivo brain imaging in small animals with sub-cellular resolution. Tilting of the objective with respect to the vertical axis facilitates positioning of the imaged animal.
Available techniques:
- fast 2-photon excited florescence imaging (using a resonant scanner)
Objectives:
Custom mesoscopic water immersion objective
- Field of view diameter: 5 mm
- Excitation NA: 0.6
- Emission NA: 1.0
Fluorescence excitation sources:
Spectra Physics InSight X3 DeepSee tuneable fs-laser (usable range: 900 nm - 1070 nm)
Detectors and cameras:
- 2x GaAsP photomultiplier tube
Emission filters:
- GFP
- RFP
Software:
- ScanImage
Other features:
- Resonant scanner for fast imaging
- Objective rotation +/- 20° from the vertical axes
- Remote focusing for fast fast focus changes
- Motorised stage
- Motorised focus
| Usage fees [SGD/hour] | LKCMed | NTU | Academia | Industry |
| 25* | 50 | 60 | 96 | |
| Location | CSB level 10, Support room 16 | |||
| Safety Notice | Requires N3 laser license | |||
| Contact | nobic.facilities@e.ntu.edu.sg | |||
*Reduced rates apply:
- off-peak hours (weekends, public holidays and 18.00 - 8.30 on weekdays): 70% of the rate stated in the table
- long-term:
- 30% of the prevailing rate applicable after 10 hours of booking/usage
- 30% of the prevailing rate (that is already the discounted rate in this case) applicable after 24 hours of booking/usage.