This motorised upright widefield fluorescence microscope features a sensitive CMOS camera for fluorescence detection and a colour camera for transmitted light. The available objectives cover a broad range of magnifications from 1.25x to 60x.
Available techniques:
- Transmitted light imaging (brightfield, phase contrast, DIC, darkfield)
- Widefield multi-channel fluorescence imaging with a sensitive camera
Objectives:
- HCX PL Fluotar 1.25x/0.04 dry, FWD 3.7 mm, CG -
- HCX PL Fluotar 5x/0.15 dry, FWD 13.7 mm, CG -
- HC PL Apo 10x/0.4 dry, FWD 2.2 mm, CG 0.17 mm (Phase contrast)
- HC PL Apo 20x/0.7 dry, FWD 0.59 mm, CG 0.17 mm (Phase contrast, DIC)
- HCX PL Apo 40x/0.75 dry, FWD 0.28 mm, CG 0.17 mm (Phase contrast, DIC)
- HC PL Apo 63x/1.4-0.6 oil, FWD 0.14 mm, CG 0.17 mm (DIC)
[FWD = free workg distance, CG = cover glass]
Fluorescence excitation sources:
- Metal-halide epifluorescence lamp
Filter sets:
Note that only 4 filter sets can be installed at a time; for currently installed filter sets refer to the booking system. You may request different filter sets when booking the microscope.- DAPI (350/50; 400; 460/50)
- FITC (470/40; 495; 525/50)
- Cy3 (545/30; 570; 610/76)
- Texas Red (560/40; 585; 630/75)
- Alexa Fluor 633 (605/50; 635; 670/50)
- Cy7 (710/75; 760; 810/90)
Detectors and cameras:
- Lumenera 5-5 CMOS grey-scale (2464x2056 pixels, 3.45 µm/pixel) with 0.55x C-mount adapter
- DFC 450 C colour camera (2560x1920 pixels, 3.4 µm/pixel) with 0.7x C-mount adapter
Software:
- LAS-X
- Micro-Manager
Other features:
- Motorised stage
- Motorised filter turret
- Motorised focus
- Darkfield condenser
| Usage fees [SGD/hour] | LKCMed | NTU | Others (Academia/Industry) |
| 0 | 15 | 25 / 40 | |
| Location | EMB 03-02Y (apply for card access) | ||
| Contact | nobic.facilities@e.ntu.edu.sg | ||