A motorised upright epifluorescence microscope with a motorised stage allowing multi-position time-lapse imaging or tile scans of large samples. The microscope is housed in a heated enclosure (e.g. for live-cell imaging).
Available techniques:
- Widefield epifluorescence imaging
- Transmitted light imaging (brightfield, phase contrast, darkfield)
Objectives:
- EC Plan-Neofluar 10x/0.3 dry, FWD 5.2 mm, CG 0.17 mm
- EC Plan-Neofluar 20x/0.5 dry, FWD 2.0 mm, CG 0.17 mm
- EC Plan-Neofluar 40x/1.3 oil, FWD 0.21 mm, CG 0.17 mm
- LD Plan-Neofluar 63x/0.75 dry, FWD 1.2-2.2 mm, CG 0-1.5 mm
- EC Plan-Neofluar 63x/1.25 oil, FWD 0.1 mm, CG 0.17 mm (Phase contrast)
- Plan Apochromat 100x/1.4 oil, FWD 0.17 mm, CG 0.17 mm
[FWD = free working distance, CG = cover glass]
Fluorescence filter sets:
- Zeiss set 49 - DAPI (G 365; 395; 420-470)
- Zeiss set 10 - FITC (450-490; 510; 515-565)
- Zeiss set 46 - YFP (490-510; 515; 520-550)
- Zeiss set 43 - DsRed (545/25; 570; 570-640)
- Zeiss set 31 - AF 568 (550-580; 585; 590-650)
Fluorescence excitation sources:
- HXP 120 C mercury metal halide lamp (intensity at sample)
Detectors and cameras:
- AxioCam MRm CCD camera, 1388x1040 pixels, 6.45 µm/pixel
Software:
- ZEN 2 (blue)
Other features:
- Motorised stage
- Motorised filter cube turret
- Motorised focus drive
- Heated enclosure
- Zeiss SVB-1 signal distribution box
| Usage fees [SGD/hour] | Academia | Industry |
| 8.4 | 16.8 | |
| Location | ABIF, SCELSE, B2 (SBS-B2n-27P) | |
| Contact | abif@e.ntu.edu.sg; nobic.facilities@e.ntu.edu.sg | |