A motorised inverted epifluorescence microscope with a motorised stage allowing multi-position time-lapse imaging or tile scans of large samples. A heated stage top chamber is available upon request.

Available techniques:

Widefield epifluorescence imaging
Transmitted light imaging (brightfield, phase contrast, DIC)

Objectives:

EC Plan-Neofluar 5x/0.16 dry, FWD 18.5 mm, CG 0.17 mm
EC Plan-Neofluar 20x/0.5 dry, FWD 2.0 mm, CG 0.17 mm (DIC)
LD Plan-Neofluar 40x/0.6 dry, FWD 2.5-3.3 mm, CG 0-1.5 mm (Phase contrast, DIC)
LD Plan-Neofluar 63x/0.75 dry, FWD 1.2-2.2 mm, CG 0-1.5 mm (Phase contrast, DIC)
EC Plan-Neofluar 63x/1.25 oil, FWD 0.1 mm, CG 0.17 mm (DIC)
Plan-Neofluar 100x/1.3 oil, FWD 0.2 mm, CG 0.17 mm (Phase contrast)
[FWD = free working distance, CG = cover glass]

Tube lenses: 1x, 1.6x

Fluorescence filter sets:

Note that only 5 fluorescence filter sets can be installed at a time; for currently installed filter sets refer to the booking system. You may request different filter sets when booking the microscope.

Zeiss set 49 - DAPI (G 365; 395; 420-470)
Chroma 49001 - CFP (426-446; 455; 460-500)
Zeiss set 10 - FITC (450-490; 510; 515-565)
Zeiss set 46 - YFP (490-510; 515; 520-550)
Zeiss set 31 - AF 568 (550-580; 585; 590-650)
Zeiss set 50 - Cy5 (625-655; 660; 665-715)

Fluorescence excitation sources:

HXP 120 C mercury metal halide lamp (intensity at sample)

Detectors and cameras:

AxioCam MRm CCD camera, 1388x1040 pixels, 6.45 µm/pixel
Point Grey Grasshopper (gs3-u351s5c-c) RGB CCD camera, 1384 x 1036 pixels, 3.45 μm/pixel, 0.63x adapter (on request)

Software:

ZEN 2 (blue)
Micro-Manager 2.0
YouScope

Other features:

Motorised stage
Motorised filter cube turret
Motorised focus drive
Stage-top incubator (on request)

Usage fees* [SGD/hour]	NTU	Academia	Industry
Usage fees* [SGD/hour]	8.4	30	48
Location	ABIF, SCELSE, B2 (SBS-B2n-27P)
Contact	abif@e.ntu.edu.sg; nobic.facilities@e.ntu.edu.sg

* Long-term discounts of 75% and 95% apply after 8 and 24 hours of continuous usage, respectively.