A motorised inverted epifluorescence microscope with a motorised stage allowing multi-position time-lapse imaging or tile scans of large samples. A heated stage top chamber is available upon request.
Available techniques:
- Widefield epifluorescence imaging
- Transmitted light imaging (brightfield, phase contrast, DIC)
Objectives:
- EC Plan-Neofluar 5x/0.16 dry, FWD 18.5 mm, CG 0.17 mm
- EC Plan-Neofluar 20x/0.5 dry, FWD 2.0 mm, CG 0.17 mm (DIC)
- LD Plan-Neofluar 40x/0.6 dry, FWD 2.5-3.3 mm, CG 0-1.5 mm (Phase contrast, DIC)
- LD Plan-Neofluar 63x/0.75 dry, FWD 1.2-2.2 mm, CG 0-1.5 mm (Phase contrast, DIC)
- EC Plan-Neofluar 63x/1.25 oil, FWD 0.1 mm, CG 0.17 mm (DIC)
- Plan-Neofluar 100x/1.3 oil, FWD 0.2 mm, CG 0.17 mm (Phase contrast)
[FWD = free working distance, CG = cover glass]
Tube lenses: 1x, 1.6x
Fluorescence filter sets:
- Zeiss set 49 - DAPI (G 365; 395; 420-470)
- Zeiss set 10 - FITC (450-490; 510; 515-565)
- Zeiss set 46 - YFP (490-510; 515; 520-550)
- Zeiss set 31 - AF 568 (550-580; 585; 590-650)
- Zeiss set 50 - Cy5 (625-655; 660; 665-715)
Fluorescence excitation sources:
- HXP 120 C mercury metal halide lamp (intensity at sample)
Detectors and cameras:
- AxioCam MRm CCD camera, 1388x1040 pixels, 6.45 µm/pixel
- Point Grey Grasshopper (gs3-u351s5c-c) RGB CCD camera, 1384 x 1036 pixels, 3.45 μm/pixel, 0.63x adapter
Software:
- ZEN 2 (blue)
- Micro-Manager 2.0
- YouScope
Other features:
- Motorised stage
- Motorised filter cube turret
- Motorised focus drive
- Stage-top incubator (on request)
| Usage fees* [SGD/hour] | NTU | Academia | Industry |
| 8.4 | 30 | 48 | |
| Location | ABIF, SCELSE, B2 (SBS-B2n-27P) | ||
| Contact | abif@e.ntu.edu.sg; nobic.facilities@e.ntu.edu.sg | ||
* Long-term discounts of 75% and 95% apply after 8 and 24 hours of continuous usage, respectively.